Breakdown of No me hables en ese tono.
no
no / not
en
in / on / by (means of transport) / about (after pensar)
hablar
to speak
me
to me / myself (reflexive) / me (direct object)
ese
that (m.sg., also esa, esos, esas, eso)
el tono
the pitch (tone); the tone (of voice); the ringtone
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