No quiero deshacer la cama otra vez.

Because Spanish often drops subject pronouns when the verb ending already shows who the subject is.

• quiero = I want
• The -o ending tells you it is yo

So No quiero deshacer la cama otra vez is completely natural.
You could say Yo no quiero deshacer la cama otra vez, but adding yo usually gives extra emphasis, like:

• I don’t want to do it again.

In Spanish, no normally goes directly before the conjugated verb.

So:

• No quiero... = I don’t want...
• No puedo... = I can’t...
• No tengo... = I don’t have...

Here, the main conjugated verb is quiero, so no comes right before it.

Because after querer meaning to want, Spanish normally uses another verb in the infinitive.

Structure:

• querer + infinitive

Examples:

• Quiero comer. = I want to eat.
• No quiero salir. = I don’t want to go out.
• No quiero deshacer la cama otra vez.

So quiero is the conjugated verb, and deshacer stays in the infinitive.

Deshacer literally means to undo.

It is built from:

• hacer = to do, to make
• des- = a prefix meaning something like un- or reverse

So:

• hacer la cama = to make the bed
• deshacer la cama = to unmake the bed / undo the made bed

More generally, deshacer can mean:

• to undo
• to take apart
• to unpack
• to ruin the arrangement of something

In Spanish, it is very common to use the definite article (el, la, los, las) instead of a possessive like mi when it is already obvious whose thing it is.

So la cama can naturally mean:

• the bed
• my bed
• the bed in question

depending on context.

This is very common in Spanish. Compare:

• Me duele la cabeza. = My head hurts.
• Voy a lavar el coche. = I’m going to wash my car.

If you specifically wanted to stress ownership, you could say mi cama, but it is not necessary here.

Yes. It is a normal, understandable expression meaning to unmake the bed or to mess up a bed that was already made.

It is the opposite of:

• hacer la cama = to make the bed

Depending on context, deshacer la cama could mean:

• taking the covers apart
• getting into a neatly made bed
• messing up a bed that had just been made

So it is a practical, everyday phrase.

Here otra vez means again or one more time.

• otra = other / another
• vez = time, in the sense of an occasion

So literally it is something like another time.

Examples:

• Hazlo otra vez. = Do it again.
• No quiero verlo otra vez. = I don’t want to see it again.
• No quiero deshacer la cama otra vez.

Yes. De nuevo also means again.

So this would also work:

• No quiero deshacer la cama de nuevo.

In many cases, otra vez and de nuevo mean almost the same thing.

Very roughly:

• otra vez is often a bit more everyday and conversational
• de nuevo can sometimes sound slightly more neutral or formal

But in this sentence, either is fine.

Yes, that is also very natural.

Structure:

• volver a + infinitive = to do something again

So:

• No quiero volver a deshacer la cama.

This is very close in meaning to No quiero deshacer la cama otra vez.

A slight difference:

• otra vez simply adds again
• volver a + infinitive expresses to do X again as a verbal structure

Both are common and correct.

Because adverbial expressions like otra vez are often placed after the main verbal idea, and that sounds very natural here.

• No quiero deshacer la cama otra vez.

But Spanish word order is flexible, so you may also hear:

• No quiero otra vez deshacer la cama.
• Otra vez no quiero deshacer la cama.

Those alternatives can sound more marked or emphatic depending on context.
The original version is the most neutral and natural.

No. Querer is an irregular verb.

Its stem changes:

• querer
• quiero
• quieres
• quiere

So the sentence uses the yo form:

• quiero

A few present tense forms:

• yo quiero
• tú quieres
• él/ella quiere
• nosotros queremos
• vosotros queréis
• ellos quieren

Notice that nosotros and vosotros do not have the stem change.

Usually no quiero most directly means I don’t want to.

It expresses lack of desire or unwillingness.

In context, English might sometimes translate it as I won’t, especially if the speaker is refusing, but the Spanish form itself is still literally I don’t want.

Compare:

• No quiero ir. = I don’t want to go.
• In some situations, that could function like I won’t go, but the grammar is still based on wanting, not the future tense.

Yes, it sounds natural in Spain.

A speaker in Spain would understand it immediately as something like not wanting to unmake or mess up the bed again.

Other natural variants in Spain might be:

• No quiero volver a deshacer la cama.
• No quiero deshacer la cama de nuevo.

But the original sentence is perfectly normal.

The opposite is hacer la cama.

• hacer la cama = to make the bed
• deshacer la cama = to unmake the bed

Example pair:

• Acabo de hacer la cama. = I’ve just made the bed.
• No quiero deshacer la cama otra vez.

This is a useful pair to learn because hacer and deshacer often work as opposites in Spanish.