No hables con el biólogo mientras trabaja.

Questions & Answers about No hables con el biólogo mientras trabaja.

Why do we use no hables instead of no hablas?
For negative informal commands (telling not to do something), Spanish always uses the present subjunctive form. If you were stating a fact (like "You don't talk"), you would use the indicative no hablas, but as a direct negative command ("Don't talk!"), it must be no hables.
Because there is a command, shouldn't mientras trigger the subjunctive (mientras trabaje)?
Not in this specific context. The word mientras only triggers the subjunctive when it refers to a future, uncompleted action (for example, "Don't talk to him while he works tomorrow"). Here, we use the indicative trabaja because it describes a reality happening right now: he is currently working.
How do we know whose action trabaja describes? Could it mean "while you work"?
In Spanish, the subject of a conjugated verb is often implied by context. While trabaja is the form for él, ella, or usted ("you" formal), we know it doesn't mean "you" here because the speaker just addressed the listener using the informal command (no hables). Switching to the formal usted mid-sentence would be very unnatural, so trabaja must refer to the third party: el biólogo.

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