Learn Spanish (Latin America): No ignoren las reglas del recorrido guiado. - Do not ignore the rules of the guided tour.

Because this is a formal plural command (an ustedes command). For regular -ar verbs like ignorar (to ignore), you form the command by swapping the usual -an ending for -en. To make it negative, simply put no in front of the verb.

Do I need to include the word ustedes in this sentence?

No, it is usually left out. The -en ending on ignoren already shows that the command is directed at a plural you (ustedes). You could say No ignoren ustedes if you wanted to add emphasis, but it is not required.

Why is it del recorrido instead of de el recorrido?

In Spanish, whenever the preposition de (of) is immediately followed by the masculine singular article el (the), they must combine into the single word del. Because recorrido (tour) is a masculine noun, we must use del.

Why does guiado come after recorrido?