No estoy cansado en absoluto.

Because estar is used for temporary states or conditions, and being tired is normally seen as a temporary condition.

• estoy cansado = I am tired
• soy cansado would sound wrong here

A quick rule of thumb:

• ser = what something is
• estar = how something is right now

So No estoy cansado en absoluto means the speaker is saying they are not tired at this moment.

In Spanish, basic negation is formed by putting no directly before the conjugated verb.

• Estoy cansado = I am tired
• No estoy cansado = I am not tired

That is the normal pattern in Spanish.

En absoluto means at all, absolutely, or not in the least, depending on context.

In this sentence, it strengthens the negation:

• No estoy cansado = I’m not tired
• No estoy cansado en absoluto = I’m not tired at all

It is a fairly strong, clear way to deny something.

Cansado is an adjective, and Spanish adjectives usually agree with the gender of the person being described.

• A man would normally say Estoy cansado
• A woman would normally say Estoy cansada

So if the speaker is female, the sentence would be:

• No estoy cansada en absoluto

Spanish often leaves out subject pronouns because the verb ending already shows who the subject is.

• estoy already means I am

So:

• No estoy cansado en absoluto = perfectly normal
• Yo no estoy cansado en absoluto = also correct, but more emphatic

You would include yo if you want contrast or emphasis, for example:

• Yo no estoy cansado, pero él sí.
  I’m not tired, but he is.

Yes, but the version at the end is the most straightforward and common:

• No estoy cansado en absoluto.

You may also hear:

• No estoy en absoluto cansado.

This is also correct, but it can sound a bit more formal or stylistically marked. For most learners, putting en absoluto at the end is the safest choice.

En absoluto is completely natural in Spain, but it sounds a little more emphatic and sometimes slightly more formal than some alternatives.

Common alternatives include:

• No estoy nada cansado. = I’m not at all tired.
• No estoy cansado para nada. = I’m not tired at all.

In Spain:

• en absoluto = clear, correct, neutral to slightly formal
• nada / para nada = very common in everyday speech

Yes, but it means something different.

• No estoy cansado en absoluto = I’m not tired at all
• No estoy muy cansado = I’m not very tired

So muy weakens the idea, while en absoluto makes the negation much stronger.

Yes. En absoluto can be used by itself to mean absolutely not or not at all.

For example:

• ¿Estás cansado?
• En absoluto.

This is a useful expression in Spanish, especially when you want to give a firm negative answer.

A simple pronunciation guide is:

no es-TOY kan-SA-do en ab-so-LU-to

A few useful points for Spain:

• estoy has the stress on the second syllable: -toy
• cansado has the stress on sa
• absoluto has the stress on lu

In much of Spain, the d in cansado is often pronounced softly, so it can sound a bit like cansao in relaxed speech, although learners should still pronounce the full form clearly.

Yes. Adding en absoluto makes the denial stronger.

Compare:

• No estoy cansado. = I’m not tired.
• No estoy cansado en absoluto. = I’m not tired at all.

So the second version sounds more emphatic and leaves less room for doubt.