V noci tramvaj do centra už nejede.

Because v meaning in/during takes the locative case here, and noc changes to noci in the locative singular.

• noc = night
• v noci = at night / during the night

This is a very common expression in Czech.

In this sentence, v noci is best understood as at night or during the night.

A natural English translation would be:

• At night, the tram to the center no longer runs.

English usually says at night, even though Czech literally uses in the night.

Czech has no articles, so there is no direct equivalent of a/an/the.

So tramvaj can mean:

• a tram
• the tram

Which one is meant depends on context. In this sentence, English usually needs the or sometimes just trams depending on the translation, but Czech does not mark that.

Czech often uses the singular when talking about a service, route, or typical vehicle in a general sense.

So tramvaj do centra už nejede can mean something like:

• The tram to the center doesn’t run anymore at night
• A tram to the center no longer goes at night

It does not necessarily mean only one specific physical tram. It can refer more generally to that tram connection.

Do means to in the sense of movement into/toward a place, and it requires the genitive case.

• centrum = center
• do centra = to the center / into the center

So centra is the genitive singular form of centrum.

Because Czech normally marks direction with a preposition plus case.

Here:

• do centra = toward/into the center

You cannot normally leave out do here, because centrum by itself would not show the meaning to the center.

Už usually means already, but in negative sentences it often corresponds to English no longer or anymore.

So:

• už nejede = no longer goes / isn’t running anymore

That is why the whole sentence sounds natural as:

• At night, the tram to the center no longer runs.

Because jet is the verb used for travel by vehicle.

• jet = to go/ride/drive by vehicle
• jít = to go on foot

A tram does not walk, so Czech uses jet:

• tramvaj jede = the tram goes / is running

In the negative:

• tramvaj nejede = the tram isn’t going / isn’t running

Nejede is:

• from the verb jet
• 3rd person singular
• present tense
• negative form

So:

• jede = he/she/it goes, drives, runs
• nejede = he/she/it does not go, does not run

Since tramvaj is singular, the verb is singular too.

Here it refers to a general timetable or regular situation, not just what is happening right now.

Czech often uses the present tense for schedules and regular services, just like English does:

• The train leaves at six.
• The tram doesn’t run at night.

So už nejede means something like it no longer runs as part of the normal night schedule.

Yes, Czech word order is flexible, but the chosen order sounds natural and highlights the time first:

• V noci = setting the time: at night
• tramvaj do centra = the subject/topic
• už nejede = the key information: no longer runs

Other orders are possible, for example:

• Tramvaj do centra už v noci nejede.
• Už v noci tramvaj do centra nejede.

But they may shift emphasis slightly. The original sentence is a very natural neutral way to say it.

It means the tram that goes to the center, so in natural English it often refers to a tram service/connection/route.

Czech often uses this kind of simple noun phrase:

• vlak do Prahy = train to Prague
• autobus na letiště = bus to the airport
• tramvaj do centra = tram to the center

So it is not just describing a random tram physically moving toward the center; it can refer to the usual service.

Yes. Depending on context, that is a very natural interpretation.

The sentence can suggest:

• the tram service used to exist at night, but not now
• at night, that tram connection is no longer available

So English translations like these can all fit:

• At night, the tram to the center no longer runs.
• There’s no longer a tram to the center at night.
• At night, you can’t take a tram to the center anymore.

In Czech, the negative particle ne- is usually attached directly to the verb.

So:

• jede = goes / runs
• nejede = does not go / is not running

This is standard Czech spelling.

Here are the dictionary forms:

• v = in
• noc = night
• tramvaj = tram
• do = to, into
• centrum = center
• už = already / anymore / no longer
• jet = to go by vehicle

And the sentence uses these forms:

• v noci = locative of noc
• do centra = genitive of centrum
• nejede = negative 3rd person singular present of jet