Learn Hardcore Spanish (Latin America): Durante la entrevista, la secretaria preguntará sobre tu última carrera universitaria. - During the interview, the secretary will ask about your last university degree.

Durante means “during.” It marks the time span when something happens. You pair it with a noun (often with a definite article) to say “during something,” e.g. durante la clase, durante el viaje.

Why is preguntará used instead of pregunta or preguntó?

Preguntará is the simple future tense (él/ella/usted) of preguntar. It tells us “she will ask.” By contrast:

pregunta = “she asks” (present)
preguntó = “she asked” (preterite/past)

Could we say la secretaria va a preguntar instead of preguntará?

Yes. Va a preguntar is the periphrastic future (near future) and is equally correct. Both sentences mean “The secretary will ask”:

La secretaria preguntará…
La secretaria va a preguntar…

Why is the preposition sobre used after preguntar?

In this context, preguntar sobre means “to ask about” a topic. You could also use acerca de (e.g. preguntar acerca de tu experiencia), but just de wouldn’t work here for “about X.”

What is the difference between tú and tu, and why is it tu última carrera universitaria?

Tú (with an accent) is the subject pronoun “you.”
Tu (no accent) is the possessive adjective “your” (informal).
Here we need “your most recent degree,” so we use tu última carrera universitaria.

What does carrera universitaria mean? Is it like “career”?

Carrera universitaria refers to your university degree or major—your course of study. It does not mean your professional career; for that you’d say carrera profesional or simply carrera in context.

Does última always mean “last” in the sense of “final”?

Última can mean “last in a sequence” or “most recent,” depending on context. Here it means “most recent” (the one you completed most recently), not “the very final degree you’ll ever get.”

Can entrevista refer to any type of interview?

Yes. Entrevista covers any formal interview—job interviews, press interviews, academic interviews, etc. If you need precision you can add a modifier: entrevista de trabajo, entrevista de prensa, entrevista académica.

If I want to be formal (usted), how would I change tu última carrera universitaria?

Switch to the formal possessive su and the matching verb form for usted:
Durante la entrevista, la secretaria preguntará sobre su última carrera universitaria.